Bolt ran the final leg of the relay at a packed Bird’s Nest stadium, hurtling through the line in 37.36 seconds after teammates Nesta Carter, Asafa Powell and Nickel Ashmeade had successfully got the baton around.
The US quartet of 100m bronze and silver medallists Trayvon Bromell and Justin Gatlin, and fellow finalists Tyson Gay and Mike Rodgers were initially awarded silver but later disqualified.
China, headed by individual 100m finalist Bingtian Su, delighted the home crowd when they were upgraded to a shock silver in 38.01sec while Canada were awarded bronze (38.13).
Meanwhile, double Olympic champion Shelly-Ann Fraser-Pryce anchored Jamaica to their second successive world title in the women’s 4×100 metres on Saturday.
Fraser-Pryce, who won the 100 metres individual title in Beijing, left Jasmine Todd in her wake as the Jamaican quartet overpowered Olympic champions the United States to clock a championship best 41.07 seconds. The Americans took silver in 41.68.
Jamaica led from the gun, Veronica Campbell-Brown producing a blistering start, which Natasha Morrison built on in the second leg passing to Elaine Thompson with Fraser-Pryce adding the final flourish to bring the baton home in style.