Bolt who is retiring after the event in London beat Great Britain’s James Dasaolu into second (10.13) but was frustrated with his start.
“I’m not fond of these blocks,” said Bolt. “I have to get this together.”
Bolt looked like he needed to work hard through the middle of the race before tapering down and shaking his head after crossing the line.
The 11-time world champion has only run under 10 seconds once this year and in what was his fourth race of 2017 over the distance he has made his own for a decade, he pointed to his starting blocks as a source of frustration.
“It’s shaky because when I did the warm up it [the blocks] pushed back,” he told BBC Sport. “It’s not what I am used to.”